Operations Management Assignment Example | Topics and Well Written Essays - 2000 words - 3

Operations Management - Assignment Example Finally, they can be used to develop systems to automate the processes they model (Gieskes, 2001). In the last case, programmers may use the process model as a guide in developing the information system or more recently, some process models can be run though process execution engines that automate the process directly from the model. A great deal of customization is often required in simulation systems modeling. From initial appearance, no system bears exact semblance to another and each time a new model is to be developed, the designer is compelled to begin from scratch. Simulation presents designers with powerful modeling tools which help in coming up with efficient systems. Interesting, even with these tools at the modelers’ disposal, they still get the feeling that they are reinventing again and again. There is always the suspicion that maybe the model they are about to design already exists or may be there already exists a model that sufficiently resembles the model to be designed. Either way, simulation provides a useful tool through which systems can be simulated. A number of techniques are often used in implementation of simulations. This project explores a specific type of simulation, a discrete event-based simulation, whereby events take place according to a schedule set on the fly as simulation plays out, with the main aim being to focus on points in time whereby interesting events take place and skip the dead spots in between the processes. For sparse simulations, where only a few events occur at irregular intervals, event-based simulation is extremely efficient. In the simulation described in this report, the dry cleaning establishment’s inner workings are modeled. Although taking note of the fact that an actual dry cleaning establishment is a physical store which not only occupies physical space, but is also complete with machinery, workers, customers, and other variables, there are further complexities than a typical model can incor porate. This simulation therefore operated a simple model but still captured the entire essential ongoing in dry cleaning process. To keep the simulation simple and reasonable, it is assumed that all garments to be dry cleaned are identical in size, weight, and material despite the fact that realistic differences are available in the process. It is assumed that over the course of time, the difference will even out. An average time is assumed for the clothing. Spreadsheet model versus ARENA modeling Discrete event modeling/simulation is largely use in generation of system predictions of states during time intervals, which are flexible in examination of what if situations. For instance, it is regularly used in evaluation of client waiting lines often referred to as a queue. The question typical of such models and simulations is how long customer will have to wait (averagely) in a line before a customer representative attends to him/her and if this wait time is too long, explore possib le ways of reducing it. Solutions may include adding servers. Modeling and simulation help explore these questions without the need to actually create and assess a physical situation as such a move could prove extremely expensive. Among the approaches often adopted in modeling are spreadsheet modeling and ARENA modeling. A model spreadsheet is defined as a ubiquitous software packages element available on many networked as well as personal microcomputer systems. It is primarily applied in

Operating System and Microsoft Corporation Essay Example for Free

Operating System and Microsoft Corporation Essay Microsoft Corporation is an American multinational software corporation headquartered in Redmond, Washington that develops, manufactures, licenses, and supports a wide range of products and services related to computing. The company was founded by Bill Gates and Paul Allen on April 4, 1975. Microsoft is the worlds largest software maker measured by revenues.[3] It is also one of theworlds most valuable companies.[4] Microsoft was established to develop and sell BASIC interpreters for the Altair 8800. It rose to dominate the personal computeroperating system market with MS-DOS in the mid-1980s, followed by the Microsoft Windows line of operating systems. The companys 1986 initial public offering, and subsequent rise in its share price, created an estimated three billionaires and 12,000 millionaires from Microsoft employees. Since the 1990s, it has increasingly diversified from the operating system market and has made a number of corporate acquisitions. In May 2011, Microsoft acquired Skype Technologies for $8.5 billion in its largest acquisition to date.[5] As of 2012, Microsoft is market dominant in both the PC operating system and office suite markets (the latter with Microsoft Office). The company also produces a wide range of other software for desktops and servers, and is active in areas including internet search(with Bing), the video game industry (with the Xbox and Xbox 360 consoles), the digital services market (through MSN), and mobile phones (via the Windows Phone OS). In June 2012, Microsoft announced that it would be entering the PC vendor market for the first time, with the launch of the Microsoft Surface tablet computer. In the 1990s, critics began to contend that Microsoft used monopolistic business practices and anti-competitive strategies includingrefusal to deal and tying, put unreason able restrictions in the use of its software, and used misrepresentative marketing tactics; both the U.S. Department of Justice and European Commission found the company in violation of antitrust laws. Microsoft is the undisputed leader in the market for operating systems (Sheremata 1997). The Microsoft Corporation has produced the vast majority of operating systems for all personal computers (PCs); moreover, operating systems that Microsoft has created are Windows95, Windows 3.1, and DOS. They also have produced the leading spreadsheet and word processors for both Windows and Macintosh operating systems. They own 85% of the market share with their office software Word, Excel, Powerpoint, Microsoft Exchange, and Microsoft Access. Not surprisingly, most of us have used some if not all of these products and/ or services provided by the Microsoft Corporation. How did the Microsoft Corporation develop into a software monopoly? For the most part, one or several combinations of the following forge monopolies: an amalgamation of smart business decisions, mistakes by competitors, and at times some shady tactics that a company might employ to become a market leader and monopoly of today. Microsoft had its beginning during the 1970s when IBM was the computer hardware giant of the industry. In 1975 Microsoft was the producer of programming languages for MIPS Altair 7500 (Conigliaro1996). In 1981, Microsoft purchased an operating system for an Intel based 8086 chip from a small company named Seattle Computer Products and redesigned its product to sell it to license it to IBM for its new personal computer (PC) (Conigliaro1996). The redesigned product was released under the name MS DOS 1.0. IBM being a monopoly at that time in the hardware department, by allowing Microsoft to provide the operating system to its PCs, then an outside source, relinquished control of the software industry to this up-and-coming powerhouse. Microsoft retained the right to license their operating system to other manufacturers and helped spur the massive IBM clone industry. Microsoft became one of the leaders in the computer industry during the 1980s with the help of technologies that help provide PCs for an information hungry age and with Microsoft providing the operating systems for a high percentage of the PCs sold. By the late 1980s, Microsoft controlled the operating system market; versions of MS-DOS ran over 80% of personal computers (Conigliaro1996). However, Microsoft did not control any of the application markets, this honor went to Lotus, which at the time had the top spreadsheet, 1-2-3 and WordPerfect had the leading word processors, WordPerfect. When Microsoft introduced Microsoft Windows 3.1 in the 1990s, it locked in Microsoft in the driver seat for what is now the software monopoly that exists. Soon after, Microsoft introduced Excel 3.0 for Windows and Word for Windows 2.0. Lotus and WordPerfect did not realize the effects that Windows 3.0 would have on the industry and did not plan ahead for the evolution (Gleick 1995). The claim has also been made that Microsoft used its control over the operating system and graphical user interface markets to help growth in the applications market. Some executives spoke of the â€Å"Chinese Wall†, that is some of the developers learned to take advantage of the operating system before other companies could who did not have the access (Gleick 1995). They were also accused of when introducing new technology called object linking embedding (OLE) in Windows, they would provide the technology to Excel 3.0 developers to incorporate it into Excel before the technology was available to other companies. Making it possible for applications on a system to work with other applications made by Microsoft before competitors could have a chance to compete and provide the same or a comparable application. With the introduction of Windows 3.1 (an update on 3.0) and Microsoft having the edge, Microsoft’s Excel, Word and Office started to dominate the application market. The dawn of Microsoft the monopoly empire was on the horizon. In the 1990s Microsoft began to diversify with its dominance over the application and operating systems it began to get into producing products for multimedia, business operation systems, and now even games and online services. They have been accused of shady business practices when they attempted to purchase Intuit, the software company that owns Quicken, the world’s most popular personal finance manager. And Microsoft’s pairing Windows 95 with The Microsoft Network, both moves are threatening to new competitors because of the barriers they create. They also generate risks to innovation and competition through the entire industry. Microsoft has used its power as the leader in the market with operating systems to grow into the power that it is now. A combination of good business tactics, regretful business choices by competitors, and finally Microsoft using its new power has made Microsoft untouchable by competitors. We have several examples of monopolies in our past as a nation, and we have found that monopolies have a tendency to stiffen innovation. With computer systems innovation is important to continue and stay ahead of the market. The example we can think about is IBM, when IBM split its business it opened the door to several new businesses that have only helped society. Is Microsoft going to help society if it is ordered to split? We can only wait and see. By making Microsoft split it will open the door to new ideas from small entrepreneurs that might have the next great operating system. # Microsoft has long enjoyed Olympian profit margins, using its monopoly power to maintain prices on its software even in tough times. But now, amid a terrible downturn and rising competition, CEO Steven A. Ballmer is shifting to a scrappier approach. He is cutting prices on a variety of fronts, from flagship Windows and Office products to newfangled Internet services. The idea is to accept lower margins in some businesses but boost overall earnings by going after a grab bag of growth opportunities. These range from expanding its share of big companies software purchases to lowering the price of Office software so consumers in emerging markets pay for it rather than pirate it. With the outlook so cloudy, were focusing on gaining share in those areas that are most critical, says Stephen A. Elop, who heads the business division. On July 13, Elop demonstrated the new Office 2010 in New Orleans. While Microsoft expects most customers to pay for the program the way they always have, less powerful, ad-supported versions will be available free on the Web. The company is also charging a monthly fee for online applications, such as the e-mail program Exchange, which is about a third as profitable as selling the software on CDs. And on Oct. 22, Microsofts new Windows 7 PC operating system will go on sale in stores for $40 less than the $240 it charged when it launched its Vista program in 2007—the biggest price cut on a new version of Windows in years. All of these moves amount to a risky experiment in price elasticity. By lowering prices, the company hopes to increase sales of existing products while making fast headway with new ones. If the company can gain enough market share to cover its massive costs in Web services and Internet search—notably, its vast data centers—every extra dollar will be pure profit. Im not saying it will be easy, says Ballmer. But we have great opportunities to grow total profit dollars. ONLY $29 IN CHINAMicrosoft is cutting the price of Office and offering the free versions of Word, Excel, and other programs to head off competition from Google and other rivals that offer similar software at little or no cost. Microsoft has so many promotions for Office that its effective price is $100, down from $150, and even lower in such countries as Brazil and India. But the experience is sparking optimism at Microsoft about the new strategy. The company says unit sales of Office surged 415% in the second half of last year. The most aggressive price cut has come in China, where Microsoft says 95% of Office installations have been pirated. Since it began testing a $29 offer in China last September, sales have soared morethan 800%. The low price was like taking firewood from under the cauldron of piracy, says Liu Tianxiang, a vice-president with Beijing Federal Software, a Chinese software distributor. He figures Microsoft has sold 80,000 copies of Office in China since the trial started. Now Microsoft intends to make the low price permanent. Not surprisingly, Microsoft continues to hunt for ways to offset price cuts. It hopes to boost the bottom line by encouraging Windows 7 software users to upgrade more often. Since the program CD will come loaded with multiple versions, users who buy the cheaper Starter edition can easily pay later to get premium features. Of course, that risks annoying users—when rivals such as Google are trying to lure customers with cheaper alternatives. But in the current economy, theres no risk-free way to stay ahead.

Picornavirus Life Cycle and Processing of Proteins

Picornavirus Life Cycle and Processing of Proteins Introduction Picornaviruses are small positive strand RNA viruses with single stranded genomic RNA capable of causing various diseases in humans (Porter, 1993). The picornavirus RNA genome has 3 poly(A) tail and a virus encoded particle Vpg at the 5 end. Unlike other RNA genome viruses, picornavirus doesnt contain a 7- methyl guanosine cap at the 5end intead they contain VPg a viral protein linked at the 5 end of the genome (Bedard and Semler, 2004). IRES (Internal ribosome entry site) is a clover leaf secondary structure at the 5 non-coding region and in the polypeptide there is a 3 noncoding region which contains the poly(A) tract required for the synthesis of minus RNA strand for RNA replication and translation. The minus strand RNa increases the efficiency of RNA replication and translation. The structural and non structural proteins are found at the polyprotein and the polyprotein is divided into three regions such as P1, P2, P3 where P1 contains the structural proteins (VP1, VP2, VP3 and VP 4) required for capsid formation and the non structural proteins P2 and P3 are required for replication and membrane rearrangement. The P2 and P3 region contains proteinase 3C and viral RNA dependent polymerase 3D (Stanway, 1990). There are 11 mature polypeptides with three main cleavage intermediates. UTR- Untranslated region; IRES- Internal ribosome entry site; VPg- Viral protein genome-linked (Lin et al, 2009). Processing of proteins The processing of proteins starts from the primary cleavage occurring between P1 and P2, mediated by viral protease 2A containing the cysteine nucleophile and it cleaves P1 and P2 leaving the viral polyprotein at cis. The P2 and the P3 precursors are separated from P1 region. For aphthovirus self cleavage takes place at the P1 domain region of the polyprotein by L proteinase at the cis whereas the L proteins in cardiovirus possess no proteolytic activity and hence in aphthovirus the cleavage of P1 is initiated by proteinase 3C. 2A proteinase also cleaves the cellular factors in entero and rhinovirus where eIF4G, a cap binding complex is cleaved and due to this even the there is a shut off in host translation. The L proteinase also cleaves eIF4G in aphtho virus during primary cleavage (Ryan and Flint,1997). There are four structural proteins in Picornavirus capsids such as VP1, VP2, VP3 and VP4 where the VP4 protein is inside of the capsid. The VP4 proteins are formed by the cleavage of VP0 precursor, late during the assembly and are modified after translation by the covalent attachment of myristic acid at the amino terminus (Cann, 1997). Picornaviruses consist of three types of proteinase L, 2A and 3C. the aphtho or F M D V polyproteins are processed by more than one proteinase. The L proteinase are available in two forms Lb pro and Lab pro. L pro possess the same function as 2A proteinase of entero and rhinovirus which cleave the host cell protein eIF4G but the major difference between L pro and 2A is that the L pro cleave in between Gly479 and Arg470 residues whereas 2A cleave in between Arg486 and Gly487. Lb pro plays a major role in substrate binding and also in shut off host cell translation like that of 2A proteinase (Ryan and Flint,1997). 2A proteinase cleaves at its own N terminus and the primary cleavage is carried at the P1 capsid protein precursor. The nature of 2A proteinase is unclear and the sequence similarities led to the understanding that 2A pro catalytic triad composed of His20, Asp38 and also an active site nucleophile of cysteine. 2A pro can be inhibited by active thiol proteinases such as iodoacetamide and N-ethylmaleimide. 2A proteinase also inhibits the host cell protein synthesis which mediated the cleavage of eIF-4G, a 220K Da polypeptide but it was later understood that the 2A pro just initiated as an activating factor for another proteolytic activity to cleave eIF-4G. Generally 2A pro shuts off the host cell translation because eIF-4G deals with cap dependent mode of translation in host cell. In poliovirus, 2A acts a trans activator of translation at IRES when host cell is not imhibited. When 2A pro was mutated it led to loss of cleavage activity in trans but not in cis, and no replication of vira l RNA was seen hence this confirms that 2A pro is required for viral RNA replication. The aphtho and cardiovirus 2A proteinase show no sequence similarity to entero/rhino virus although 2A protease are similar in size. The primary cleavage of hepatovirus and echovirus does not take place by the cleavage of 2A proteinase and the 2A protein in hepatovirus and echovirus showed no proteolytic activity (Ryan and Flint,1997). 2B and its precursor 2BC is a viral protein consisting of two hydrophobic regions with ÃŽ ± amphipathic a-helix domains leading to the alteration of membranes in the infected cells. The virus induced vesicles are formed when 2B and its precursor 2BC enter into the host membrane of Golgi and ER complex by altering the permeability of plasma membrane to form virporin complex. Once the 2B and the precursor 2BC enters the host there is an imbalance in Ca2+ homeostatis mechanism and blocks protein transport from ER to Golgi and also initiates anti-apoptosis property. The 2B protein also blocks the activation of IRF-3 in Hepatitis A virus through which the cellular IFN-ÃŽ ² gene transcription is inhibited so that there is no harm to Hepatitis A virus in the host. (Lin et al, 2009). The vi ral RNA was anchored for the spatial arrangement required for replication by the 2C protein. The mutagenesis experiments confirmed that the 2C proteins are involved in strand separation of viral RNA while replication (Porter, 1993) Secondary cleavage: The secondary cleavage is carried out within the viral proteins and hence it is mediated by 3C proteinase which plays a very important role in protein processing and RNA replication. The replication proteins are generated within the P2 and P3 precursor proteins when 3C self cleaves at the P3 region of the polyprotein. 3C proteinase or the 3CD precursors cleaves the poly(A) binding protein to inhibit viral translation during late poliovirus infection. 3C also cleaves the host cell protein required for transcription. The key processing step for the viral protein processing cascade is initiated by 3C proteinase. The 3CD proteins also play a major role in carring out important functions during RNA replication (Bedard and Semler, 2004). The major function of 3C proteinase in secondary cleavages is that it process the capsid and the replicative protein precursors. The processing of capsid in poliovirus is done by 3CD proteinase and not 3C proteinase. The 3C proteins are also used to cleave various number of host cell proteins such as histone H3, transcription factor IIIC, TATA binding protein and microtubule-associated protein 4. The 3CD proteinase depends on the host cell protein EF-1 ÃŽ ± and the host cell factor is replaced by 3CD forming 3AB:3CD proteinase complex to bind at the 3 end of the poliovirus genome (Ryan and Flint, 1997). The 3A protein inhibits the cellular protein function and also presents the membrane proteins during viral infection. When the poliovirus 3A protein was mutated, the uridlylation of VPg was affected and also inhibited the viral RNAs plus strand initiation (Porter, 1993). 2B is a viral protein which is required for virion release by altering the cell membrane increasing permeability which is required for poliovirus RNA replication. 2C proteins and its precursor 2BC is necessary for the re-arrangement of intracellular membranes and also for the viral induced cytoplasmic vesicles. 2C binds with the minus strand of poliovirus RNA at the 3 non coding region and hence it plays a role in positive RNA viral strand synthesis and also in minus strand RNA synthesis. 3C and 3D protein helps in immune response interference and also in viral RNA replication. 3A protein is very much important because these proteins help the picornaviruses to escape from MHC-I ( Major histo compatability) expression and intracellular membrane transport by inhibiting both the MHC-I and intracellular membrane transport of the host cell. 3B protein also called as VPg is linked to the 5 end of both the positive and negative strand RNAs. 3AB, 3C, 3CD and 3D are required during the proces s of assembly in replication and also initiates viral RNA polymerase 3D and self cleavage of 3CD. 3C and 3D are involved in binding viral RNA, protein processing and RNA replication. The cloverleaf structure of poliovirus virus and coxsackievirus consist of stem loops in which the viral polymerase precursor, 3CD binds to the stem loop I. 3CD also binds with the host cell protein poly r(C) binding protein 2 (PCBP2) to help only in RNA replication. 3D is responsible for VPg uridylylation and RNA chain elongation while synthesising viral RNA because it contains RNA dependent polymerases and even tends for error prone and mis-incorporation of 1-2 nucleotides per replication (Bedard and Semler, 2004). VP1, VP2 and VP3 are the three larger capsid proteins folded into eight stranded antiparallel ÃŽ ² barrels and a small fourth protein called VP4 is located inside the capsid. The 3C protease cleaves VP3, VP1 and VP0 at the P1 region. The amino termini of VP0, VP3, VP1 initiates the assembly of the virion particle. VP0 peptides are cleaved into VP2 and VP4 at the final stage of processing and assembly. VP4 and VP2 are adjacent to each other at their ends when cleaved. The infection of the host is initiated when the capsid proteins bind to the receptor on the host membrane. ICAM-1 (intercellular adhesion molecule 1) is the receptor molecule for the major rhinovirus which binds the cell to adjacent substrates. Poliovirus receptor molecule is an integral membrane protein consisting of one variable and two constant domains and this receptor molecule attaches to the host cell to initiate replication process. The general receptors used by the various picornaviruses are poliovirus receptor f or poliovirus attachment, ICAM-1 receptor for major rhinovirus, LDL-R for minor group rhinovirus, CD55 or DAF receptor for some echoviruses and group B coxsackie B1-B6 receptors. A deep cleft known as canyon is formed by flanking the monomers, VP1, VP2 and VP3 which helps the virus to escape the immune response by the host cell. The interaction of capsid proteins with the intracellular host factors affects the induction of apoptosis (Lin et al, 2009). Host cell shut off mechanism The mRNA of picornavirus is uncapped and hence the translation takes place by directly introducing ribosomes at internal ribosome entry sites (IRES). The cleavage of eukaryotic translation initiation factor, eIF4G by 2A protease inhibits the cap dependent mRNA translation of the host cell. When poliovirus is introduced into the host it inhibits the host cell translation leading to apoptotic cell death. The eIFGII is more resistant to the infection of poliovirus than eIFGI. The death inducing proteins encoded by cellular mRNA were translated by cap independent translation leading to apoptotic death. The cleavage of eIF4GI by caspase 3 also induces apoptotic cell death but differs from poliovirus 2A protease process. Severe inhibition of translation leading to apoptosis is seen by both the caspase 3 activity and 2A protease. IRES elements in mRNAs encode proteins which regulate apoptotis. Even poly (A) protein and dystropin protein are also cleaved by 2A protease.. Hence, the cleavage of poly(A) binding protein by 2A protease cause apoptotis and the cleavage of dystrophin protein can induce apoptotic process due to cytoskeleton disruption (Goldstaub et al, 1999). The enterovirus 2B protein suppresses apoptotic pathway of the host cell by controlling intracellular Ca2+ homeostatis. The apoptotic responses are initiated by the 3C and 2A proteinase to inhibit cellular transcription and cap dependent translation. When the 2B proteins are suppressed by caspase-3 activation it leads to apoptotis (Campanella et al, 2004). The cellular mRNAs encode for the death inducing proteins which are translated by cap independent translation. When eIF4GI and eIF4GII are cleaved by 2A protease it leads to apoptotic death because it inhibits cap dependent translation. The 2A proteins also cleave the poly(A) binding protein and the dystropin protein which leads to apoptotis through a translational mechanism (Goldstaub et al, 2000). The eIFE is the component of cap binding complex of cap structure at 5 end of mRNA. The 40 S ribosomal subunit checks at the 5 non coding region until it finds the initiating codon or the the authentic start codon AUG and then sends a signal to the 60S to form a complex. For translation to occur in mRNA the 5 non coding region with the cap end should bind with the eIFE and once it binds to the cap the 40 S ribosomal subunit scans for the authentic initiation or start codon AUG and once it finds the AUG codon it gives a signal to 60 S ribosomal subunit to form a complex with 40 S and initiate the translation along with initiation factors (Bedard and Semler, 2004). In picornavirus the 5 end of mRNA in the non coding region is not capped and hence to initiate translation, the cap independent mechanism is required. Hence in picornavirus, the 40 S ribosomal subunit scans for ribonucleo protein complex at 5 non coding region and initiation take place to recognise authentic start codon. The eIF4G is cleaved by viral proteinase 3C and 2A which shuts off the host cell translation (cap dependent) and also cleaves poly(A) binding protein (PABP)and hence it inhibits the host cell translation. When the host cell is infected by Foot and mouth disease virus (FMDV), the eIF4G subunit which is responsible for the cap dependent translation of the host is cleaved by the L protein and for the Encephalomyocarditis virus (EMCV), the cap dependent translation of host is blocked by a repressor protein 4E-BP1 which binds at the 5 cap region and hence the repressor protein 4E-BP1 blocks the binding of eIF-4E which is the cap binding subunit and hence the host translation is shut off. The Hepatitis A virus (HAV) does not shut off the host translation because it requires eIF4G for its translation (Bedard and Semler, 2004). All picornavirus consist of internal ribosome entry site (IRES) and are mapped to the 5 non coding region. There are four types of IRES seen in picornaviruses based on the RNA secondary structures. The enterovirus and rhinovirus comes under the Type I IRES. Aphthovirus and cardiovirus comes under the Type II IRES and the Hepatitis virus comes under the Type III IRES. The porcine reschovirus comes under the Type IV elements. The IRES mediated translation is initiated by two factors Canonical initiation factors and IRES trans-activating factors. Canonical initiation factors to initiate IRES mediated translation: The IRES elements of poliovirus and EMCV are similar and require the initiation factors to be primed at the 40S ribosomal subunit. The IRES mediated translation is initiated by certain canonical factors such as eIF4G and eIF4B to bind to the viral RNA and also certain other subunits such as eIf3 and eIF2 to pre-bind to the 40 S ribosomal subunits. The IRES translation of poliovirus and EMCV is promoted by the poly(A) binding protein (Lin et al, 2009). Noncanonical initiation factors to initiate IRES mediated translation: The polypyrimidine tract-binding protein (PTB) is a 57 KDa mRNA splicing factor which increases and promotes the activity of IRES in poliovirus. The molecular switching from translation to the replication of poliovirus is done by the proteolytic cleavage of PTB. The PTB also functions as RNA chaperon by stabilizing the type II IRES of FMDV and EMCV. Lupus autoantigen (La) is a 52 KDa of nuclear protein which binds to certain distinct parts of HAV IRES and with small interfering RNA and hence the HAV IRES translation and replication is suppressed. Poly(rC) binding protein (PCBP2)bind to the type I IRES of picornavirus and hence it leads to internal initiation of translation in type I IREs elements. The heterogenous nuclear ribonucleoprotein A1 (hnRNP A1) is an RNA binding protein which binds to the 5 UTR of HRV2 and regulates transcription (Lin et al, 2009). The picornavirus infection has lowered the level of host cell transcription and hence there is a increase in the number of viral RNA molecules. The nuclear localization signal (NLS) present in the poliovirus 3D protein targets the 3CD precursor to the nucleus for autocatalytic final maturation and allow the release of 3C protease (Bedard and Semler, 2004).) Picornavirus RNA replication: The new positive and negative strand viral RNA are synthesised by using the viral encoded RNA dependent RNA polymerase 3D. The RNA dependent RNA polymerase 3D acts as a protein primer and forms VPg-pU-pU which initiates the viral RNA replication and the process is known as VPg uridylylation. When VPg uridylylation is done, the poly(A) tract at the 3 end acts as an initiation site for the synthesis of negative strand RNA synthesis. The negative strand developed act as a template for the synthesis of new viral positive RNA strand by cap independent translation. Many or numerous copies of positive viral RNA strand can be synthesised from a single negative strand. In picornaviruses, there are numerous RNA sequences and secondary structures within the 5 non coding region which are essential for the RNA replication. The viral protein 3CD and the host protein, PCBP binds at the 5 cloverleaf structure of the non coding region. The cellular host protein PCBP binds to loop b and the 3CD binds to loop d of the 5 cloverleaf structure. A ternary complex is formed when 3CD and PCBP2 binds with the cloverleaf structure along with the viral RNA to form RNA replication. When PCBP2 was depleted, there was poor RNA synthesis in poliovirus and hence it was recognised that PCBP2 play a vital role in RNA replication. PABP is a cellular protein which binds to the poly(A) tract at the 3 end of viral RNA interact with 5 cloverleaf structure of PCBP2 and the viral protein 3CD. When the PABP at the 3 end interact with PCBP2 at the 5 end, the viral RNA may interact and hence there is an initiation of replication process. Even the 3AB and 3CD proteins interact at the 5 cloverleaf structure to each other there by initiating viral RNA replication. The cis-acting replication element (cre) was found within the coding region of picornavirus genomic RNA which is required for viral replication and viability. In aphthovirus the cre structure is at the non coding region of the 5 end. The cre sequence AAACA was found in the conserved hairpin structure at the coding region of picornaviruses required for RNA replication. The cre elements act as a binding site for viral replication proteins and also as a template for VPg uridylylation. At cre element, VPg is more efficient than at the poly(A) tract and uridylylation on cre structure leads to only positive strand RNA synthesis and for uridylylation and replication initiation of the minus strand uses the poly(A) tract. The interaction between 3CD with cre and cloverleaf RNA may arrange the viral RNA structurally for efficient RNA replication. The 3 NCR and the 3 poly(A) tract forms the site of replication for minus strand RNA synthesis. The poly(A) tract at the 3 end functions for virus viability, impart stability and also for the efficient RNA replication by interacting with viral RNA at the 5 end (Bedard and Semler, 2004). The negative strand is first formed by using the VPg protein primer and the VPg plays an important role in replication. The enzymatic activity of 3D polymerase has lead to VPg uridylylation which covalently couples with the tyrosine residue in the VPg protein. The 3 poly (A) tract template may also involve other host proteins for replication. The 2C bind at the 3 end the negative strand showing ATPase activity. The RNA polymerase 3D unwinds to develop viral RNA synthesis (Bedard and Semler, 2004).

The Purpose of Education Essay -- Philosophy of Education Teaching Tea

The Purpose of Education In attempting to define the true purpose for education, people tend to focus on goals, which differ from one person to another. Most Americans approach education as a means to end. Many focus on the possible social, economic, and political benefits that can come from furthering education. While education certainly is capable of producing amazing results in these specific physical realms, perhaps we shouldn’t focus on its material goals, but rather on its mental impact, which is where reason is derived from. Through the use of reason in the form of education, we will be better able to come to terms with ourselves and our environment, which will bring us closer to reaching our full potentials as human beings. It is our advanced intelligence which makes us unique in this world, and perhaps it is the use of reason which will eventually reveal our true purpose of being. The first step toward reaching our full potential deals with gaining a better understanding of ourselves. Simply by being honest with ourselves and using our given ability to reason, we will be better able to come to terms with what is truly our purpose of living. By concerning ourselves with the absolutes of human nature we will be much closer to reality, opposed to an approach that is simply based on already established ideals. Armed with reason and a good understanding of our own human nature we will be more prepared to understand and react to our ... The Purpose of Education Essay -- Philosophy of Education Teaching Tea The Purpose of Education In attempting to define the true purpose for education, people tend to focus on goals, which differ from one person to another. Most Americans approach education as a means to end. Many focus on the possible social, economic, and political benefits that can come from furthering education. While education certainly is capable of producing amazing results in these specific physical realms, perhaps we shouldn’t focus on its material goals, but rather on its mental impact, which is where reason is derived from. Through the use of reason in the form of education, we will be better able to come to terms with ourselves and our environment, which will bring us closer to reaching our full potentials as human beings. It is our advanced intelligence which makes us unique in this world, and perhaps it is the use of reason which will eventually reveal our true purpose of being. The first step toward reaching our full potential deals with gaining a better understanding of ourselves. Simply by being honest with ourselves and using our given ability to reason, we will be better able to come to terms with what is truly our purpose of living. By concerning ourselves with the absolutes of human nature we will be much closer to reality, opposed to an approach that is simply based on already established ideals. Armed with reason and a good understanding of our own human nature we will be more prepared to understand and react to our ...

Media in Court Cases

Media in court cases has many effects. One of which is the possibility that the Medias opinion may result in tainting the jury with unproven facts. As humans we make decisions based on how we perceive the world and the information we have on decision we are going to make. Pre-Trial Publicity â€Å"Due to extensive media coverage, jury selection in a high profile case can be extremely difficult. Jurors will likely have developed some biases about the case based on the media coverage to which they have been exposed†( â€Å"Media Influence In Capital Cases†,  2011).Under the 6th amendment you have the right to a fair trial by an impartial jury of your peers. That becomes extremely difficult and possibly impossibly when the media open a flood gate of â€Å"facts† and opinions before the case has even begun. In some instances they have to relocate to court case to an area where the media coverage of the case has not been so rampant just too find impartial jurors. Wh ich just increase the cost of the court case. Not only can media influence the opinion of the jury but also the public.During the Casey Anthony case the media had a barrage of legal professionals condemning this women before the final verdict was concluded. TIME magazine called it† The Social Media Trial of the Century† (Varma,  2011). No matter what the outcome of the case that person will never have a normal life. â€Å"According to data  from  NM  Incite,  64  percent  of  people  on  Twitter  disagreed  with  the  Ã¢â‚¬ not  guilty†Ã‚  verdict,  while  only  1% agreed (35% were neutral)†( Varma,  2011).Because of media allowed in courts this woman is considered guilty by 64% of people, even though she was found not guilty. References Media Influence in Capital Cases. (2011). Retrieved from http://www. capitalpunishmentincontext. org/issues/media Varma, A. (2011). Twitter: Only 1% Think Casey Anthony Innocent. Retrie ved from http://www. socialnomics. net/2011/07/08/twitter-only-1-think-casey-anthony-innocent/

Discuss the Difficulties in Seeking to Adopt a Common Social Policy and Social Welfare Agenda Among the E.U. Member States.

Assignment 2-Take Home Exam (Question 3, 5 and 6) Question 3 Discuss the difficulties in seeking to adopt a common social policy and social welfare agenda among the E. U. member states. Introduction A social policy is a public policy and practice in the areas of health care, human services, criminal justice, education, and labor. (Malcolm Wiener Centre) In European Union, it has passed a long way to seeking adopt a common social policy and social welfare agenda among the E. U. member states. Caune et al has summarized the process of social policy into three steps followed by the milestone of EU.First stage was to create a common market and keep the national welfare policies. During the first stage E. U. did seek to establish a certain policy, such as freedom of movement for workers and freedom of establishment and equal pay and rights for migrant workers. The second stages was Maastricht treaty that creating Maastricht criteria as new economic policy regime and established ‘sof t law management’. The thirds stage was focus onwards coordination and competition of national welfare policies. The treaty of Lisbon which is the recently moment in E.U. social policy, it defines E. U. seeks to assess the significance of the poverty/social inclusion open method of co-ordination in terms of what it indicates about the EU’s engagement with social policy. From the historically, EU was did a lot of works to creating social policy and social welfare agenda. But E. U. still faces many difficult to making a common social policy among E. U. states. Furthermore, this essay will mainly discuss on the difficult in seeking to adopt a common social policy and social welfare agenda among the E. U. ember states which are based understand and analyzed the history and concept of E. U. social policy. Discussion From the three stages of form a social welfare system, we could found European Union has really well social welfare systems as an example for the rest of the wo rld. It has maintained social equality among EU members which defend weaker market participants and guarantee them acceptable standards of living. However, EU is now face great challenges, such as rapid growth in EU expansion and integration, growing competition among member states for investments.Most of them are now becoming difficult to a adopt a further common social policy EU, such as increasing about personal expectancy, population migration process, growing income inequality and the existing social exclusion. These difficulties are mainly coming from two sources which are national and European level. If EU aims to form a common social policy, they will firstly facing a problem of different social policies pursued by member states. Rutkauskiene indicated that there is† no unanimous opinion about all existing social policy in EU. (Rutkauskiene, 2009) Every member states have their social policy depends on different typology, such as Mediterranean model and antipodean mode l. These different social policy models in the place which lead EU faces a huge challenge-too many different social policy model in the members will hinder the process of adopt a common social policy in EU. One of the objectives of common social policy is maintain social equality among EU member state but each member state has a different economic situation that leading to different budget on social welfare expenditure.Hence, there will be conflict between different countries investment on the social welfare. One of the example are from the EU integration process, employee are free to move to a low cost countries and also employee from poor countries can move to a member state that has a better work condition. The enlargement or integration of European has becoming one of the difficulties in order to adopt a common social policy in EU because it has direct on the social issues, such as unemployment rate and fair work rights.Traser describe enlargement had already, in 2004, caused pu blic anxiety about large numbers of low-skilled and semiskilled workers from the new Member States seeking both employment and benefits in the EU-15, and displacing national workers with cheap labour. (Traser, 2005) The issues about free movement of employee is only one example about the differences of economic situation between member states but it can be a main difficulties for EU to adopt a common social policy because the members state are only stand for their own country and competing with other member states.In the European level, EU are also did a lot of work trying to leading member states participate on the process of adopt a common social policy but it is difficult as well. Since the Maastricht Treaty a concept of ‘soft law’ management measures are used to implementation of the EU activates. This has given to the control measures that are based on voluntarism, education and the sharing of best practices. (Rutkauskiene, 2009) In other world, member’s sta te is voluntary participation in an exchange of information or action.The European council collected all these soft measurement 2000 in Lisbon and give them a name of ‘Open Method of Coordination. (OMC)† (European Council Web) In the European council website explained OMC- set goals are monitored and supervised, best practices are shared and there is a scope to share. (European Council Web) But there are one important feature of the OMC is that goals and achievement are established at the EU level, while the measure and practice to achieving them are left for national governments. Many scholar are debate the disadvantage of this method.OMC is the lack of obligation to implement any agreements, and the lack of sanctions for failing to meet any obligations (Szyszczak, 2006). In other words, EU did not give in to any suggestion about policy to national government, and national government did not need to adequately orient their active measures according to OMC goals. Moreove r, each member states can present their own conclusions on the certain policy areas in their national actions plans, such as pension and health care area. Rutkauskiene has found a greatest number of faults in pension’s area caused by OMC. Rutkauskiene, 2009) Everyone is too different in their personal needs and clamming to adequate for all is not feasible. So a government policy should be set a minimum pension sum to be guaranteed and set of this agreement among EU member states. In other worlds, it is necessary that guidelines for changes in indicator evaluation are set, thereby blocking the way for different understanding about social affairs. From the different argument on OMC policy we can it was mainly established a principle of turning into coordination among EU member state but it facing difficulties turning this policy into an operational manner.Vandenbroucke state the post challenge of Lisbon treaty is EU need an operational social policy. (Vandenbroucke, 2002) Unfor tunately, according to the discussion that the current OMC policy has some disadvantaged that made difficulties for EU to adopt a common social policy. Conclusions This essay has started with an introduction milestone of adopt the EU social policy. The difficulties in adopt a common social policy among EU member states have been compounded furthermore by the fact that large number of state in EU and each of them implementing a different social programs and social policy measures.Then we look on how European Union to dealing with this difficulties of great variety in the social policy systems. We have been chooses the current model to coordinate of social affairs in 2000 at the signing of Lisbon strategy which are Open Method of Coordination. Based on the analysed from different academic literature, â€Å"the main shortcomings of the OMC were identified as the lack of obligation and no sanctions for failing to carry out the activities set out in the agreements reached. (Rutkauskiene , 2009) Hence, the inefficiency of current policy is other main difficulties in adopt a common social policy. At the end, the process of adopt a common social will be forward in the future and the difficulties are also coming continuously at different stages. Reference Arnaudova, F. Z. L. A. A. (2011). Growth, well-being and social policy in Euroep: trade-off orsynergy. European Social Policy Centre Concuil, E. , from http://ec. europa. eu/invest-in research/coordination/coordination01_en. htm Daly, M. (2006). EU Social Policy after Lisbon.Queen's Univeristy, Belfast. Malcolm Wiener Center for Social Policy. Retrieved 10 Jan 2013, from http://www. hks. harvard. edu/centers/wiener Palier, P. R. G. a. S. J. a. B. (2011). The EU and the Domestic Politics of Welfare State Reforms. England. Rutkauskiene, L. (2009). Problems in the formation of the common EU social policy: Vilnius Univeristy. Szyszczak, E. (2006). Experimental Governance: The Opend Method of Coordination. European Law Jou rnal. Traser, J. (2005). Report on the free movement of workers in EU-25: who's afarid of EU enlargment? Brussels:European Citizen Action services. Vandenbroucke, F. (2002). The EU and Social Protection: What should the Euroepan Convention Propose. Retrieved from http://econstor. eu/bitstream/10419/44291/1/644397675. pdf Vobruba, G. Debate on the enlargement of the Euroepan Union. University of Leipzig. Question 5 the single market is the fundamental economic underpinning of the EU. Discuss why  this single market is problematic in the EU with regards to the digital technology sector Introduction The Europe commission in a 1985 white paper launched the single market programme.The main purpose of single market is ‘seeks to guarantee the free movement of goods, capital, services, and people – the EU's â€Å"four freedoms† – within the EU's 27 member states. †(European Commission Web) It was launched as the fundamental economic integration of the EU. It creates large benefit to the enterprise and EU-citizens. The European commission and the EU’s executive arm, has target ‘energy, digital and transport sectors as priorities for depending market integration. †(Egen) In relating to the digital technology sector, the world economic is now more deepening on the digital technology.A 2010 study commissioned by the European Policy Centre from Copenhagen Economics showed that an integrated European Digital Single Market (DSM) would lead to an increase in GDP of at least 4%, with concrete benefits for consumers and citizens. (Economics, 2010) European commissions have already been set in motion about importance of digital single market. For example, Monti report had already highlighted the importance of developing the digital single market, which was also reflected in the digital agenda and the single market act.However, there are still many problem existed in the EU with regards to the digital technology sector. This e ssay will outline of the reasons of why this single market is problematic in digital sector, which are mainly because of less enforce on inappropriate regulation in the member states and cost effectiveness and differences in provision of the infrastructure and ‘old national monopolists’. Discussion In Pablo’s report, which has summaries the European commission need to work more on to build trust and confidence in digital single market.Echeverria has indicated that â€Å"European Commission need to stresses that the consumer rights directive marked an important step forward in terms of increasing legal certainty for consumers and businesses in online transactions, and today constitutes the main consumer protection instrument for online services. † (Echeverria) A single market strategy will require a higher level of legal regulations in regarding to issues such as cybercrime, data privacy and spam while ensuring free movement and the possibility of transacti ons on the internet.Otherwise this single market will be a problematic in digital sector because of the existence of a patchwork of different legal provisions and barely interoperable standards and practices. Also the consumer can’t access the full benefits from this strategy if this regulation is poor. European commission’s report of building the digital single market has identified more and more pollution is using the digital technology now. (Commission, 2011) Peoples are now using more internet service to making a convenience life, such as the online cross border trade.Moreover the digital single market will allow citizens to have access throughout the EU to all forms of digital content and services. So in order to creating single market digital sector, if people are not use digital service in a safety environment then there will be a data protection problems. The other reasons of why  this single market is problematic in the EU with regards to the digital technol ogy sector, which is cost effectiveness. In Zuleeg’s report has determine that a single digital market will require large scale investments in fixed and mobile networks, with much of this investment needing to come from private operators. Zuleeg, 2012) Especially in the European finical crisis period, Europe government and private operator will need to have spent more to support this investment by developing new investment vehicles and guarantees. Michelle Egan also defines a digital single market is a long way of investment and still have many barriers now. (Egan) But the single market in digital sector will improve productivity and contribute to increasing Europe’s medium to long term competiveness. It also brings out benefit beyond the economic which it can help some societal problem, such as fragmented labor market and environment problems.According to all of this facts, we can finding the single market can bring large benefit to citizens and social but it will nee d to put extra investment by government and private operator. So this single market will bring out a conflict between internal users and external stakeholders because of cost effectiveness. A study by Copenhagen economic has list out â€Å"there is a range of national and international operators, totalling close to 100 mobile operators. â€Å"(Copenhagen economic) In the Australia digital sector there are mainly one operator provide the most mobile and internet infrastructure which are Telstra.The digital sector is fragmented in European compared with other countries. The most of the digital companies are competing on a national scale instated of across borders. None have continent-wide operations and provide difference in provisions of infrastructure. One of the example is there are still less operator can provide mobile service across borders and also with a high roaming fees. However the single market strategy in digital sector is trying to integrate these companies into one gr oup.This single market strategy may become problematic in the digital technology sector because a fragmented supplier industry may hamper certain developments. From the overall finding, we can operator is the main stakeholder with a large impact on the digital sector. The study by Copenhagen has further explained this fact as â€Å"a lack of market consolidation with ‘old national monopolies’ keeping their strong position in local markets due to government protection in the past. â€Å" The operators are stress on their profit and ignore the importance of single market.One of the major benefits of European single market is increasing competition, leading to lower prices and better welfare for consumers and society as a whole. But the operator has main power in the national market and can refuse price convergence. Conclusion At the end, the single market in digital will have large impact on European economy either in public sector or employee or consumers or producer s ides. But according to the nature of digital technology sector which is fragmented industry and investment barriers so the single market has being a problematic in this industry.Reference Completing the internal market White paper from European Commission to European Council (1985). Brussels. Commission, E. (2011). Building the digital single market-cross border demand for content services. Echeverria, P. A. On completing the digital single market Economics, C. (2010). The conomic impact of a european digital single market. Egen, M. Twenty years after the completion of the EU's single market programme, member states have still not eliminate all barriers to trade. London: The london school of economic and politcal science. Zuleeg, F. (2012).A digital single market by 2015. eSharp. European Mobile Indsutry Obeservatory. (2011) Monti, M. (2010). A new strategy for the single market. â€Å"The Single Market†. Europa web portal. http://ec. europa. eu/internal_market/index_en. htm. Retrieved 03 January 2012. Question 6 what are the problems to be encountered in forming a European sense of identity among the citizenry of the EU? Introduction A sense of a national identity is â€Å"the person's identity and sense of belonging to one state or to one nation, a feeling one shares with a group of people, regardless of one's citizenship status. (Smith, 1993) Usually, these are nation-states but it also can implied an entity group of European Union. McCormick writes sense of European identity as â€Å"a related term of Europeanism refers to the assertion that the people of Europe have a distinctive set of political, economic and social norms and values that are slowly diminishing and replacing existing national or state-based norms and values. †(McCormick, 2010) Johan Borneman indicates the practices of Europeanization in term of languages, money, tourism and sex and sport. Borneman 1997) European Union are getting practice on this through the creation of the European single market, the expanded the European Union from twelve members in 1985 to twenty-seven members in 2007 and link the legislative and policy frameworks of EU with European identity. As we explained before EU has a long history of this integration process but there are still many problems encountered in forming a European sense of identity among the citizenry of the EU. There are especially in some countries are having this problems such as British.Moreover, this essay will discuss on the main problems that are in forming a European sense of identity among the citizenry of the EU. It also will consider some examples in English. Discussion Medrano has summarized the main problems into three section which are â€Å". 1) conflated behaviour in referenda on reform treaties of the European Union, support for European integration, and identification with Europe, 2) conflated different dimensions of European identity, and 3) failed to unpack the various meanings that citizens a ttach to the idea of identification with Europe† (Medrano, 2010)In the detail, the first problems are mainly concerned on the public debate on European identity. Many people see no opportunity to influence supranational decisions effectively because there are lacks of intermediary actor primary covering European issues. In the public, the media or journalists are both have lack of supporting on EU news. Vreese said â€Å"It is difficult to ‘sell’ an EU story. † (Vreese, 2004) Medrano has asked many journalists do you agree you play an important role in ‘crating a European identify’. (Medrano, 2010) The most of them unanimously agreed that the answer should be ‘NO’.Some journalists believe their role is to create engagement and interest and not to influence identity. One of the examples is in British, the public opinion is divided and the country is becoming the most of skeptic members in EU with regards to EU policy of common curre ncy and the enlargement. There is other problem influencing public opinion about Europe, such as difference in social-demographic characteristics. Most of researcher has find men being more supportive of Euro-pan integration and higher levels of education are associated with being more positive towards the EU.The second problems listed by Medrano, can be described as there are having many dimensions of European identity either by national or citizen. If there are too many dimensions of European identity that will results a lack of precision use in the use of the sense of European identity. The official dimensions of European identity is a precondition for a democratically legitimise European Union with feeling of belonging together of the people living in the member states, including the awareness and the support of common values, achievements and aims.But in related to a real case the European identity is far lagging behind national identity. Fukuyama has given one example of Franc es created a strong national identity by built around the French languages. (Fukuyama, 2012) In order to compared within the EU’s dimension, EU are more stress on political and policy identity but the nation’s dimensions are more focus on culture and social level. EU has 27 members within different culture and religion. These countries have already built on different level of national identity.EU is now trying to integrate this national identity into one common identity which is European identity. So EU needs to conflate different dimension of European identity. The third problems is failed to consider the citizen’s ideal about European identity. The EU defines concept of European identity are most physically based, such as free movement of goods and service. But the officials should to promote a sense of belonging to Europe citizens emotionally. Medrano stated there are lacks of identification with Europe among citizens are mostly interested in the emotional di mension of identification. (Medrano,2010)Besides of Medrano’s measured three problems, there are still many other problems in order to create a sense of European identity. One of these problems are EU has less use any knowledge or instruments of identity policy to deliver the sense of European identity, such as education. Walkenhorst writes â€Å"without a sense of commitment and knowledge of citizenship rights the European peoples cannot establish a democratic identity in the sense of supporting the EU as a legitimate political system†. (Walkenhorst, 2004) EU also will not being able to demonstrate its benefits for the European citizen without using an instrument of identity policy.For instance, provide more education or program on spread the sense of European identity will also help to avoid the problems of different religions. EU has different religions identity, such as Christian and Muslim. The concept of European identity need to consider the ideal of multicultur alism and democracy. Conclusions This essay draws an analysis of the problems encountered in forming a sense of European identity which are based on an understanding what is a sense of European identity and how could generate a sense of identity.Since the firstly forming a European Union, EU are trying to creating a sense of European identity. A sense of national or regional identity is an emotionally feeling belongs to a group. EU did a lot of work that letting people are physically feeling of European citizen, such as free movement of people and goods. One of the examples, are Eurostar given people are more mobility in traveling around European. However, EU is now facing problems on ignored the citizen’s emotionally feeling of European identity and conflicts of different dimension about European concept of identity.Each member state and citizens has different dimension about European identity. The best way to solve this problem is using accurate instrument to spread the ide al of European identity, such as education and media. But the fact is there is lack of use media and education that results a problems in forming a sense of European identity. Reference Adrian Favell, E. R. , Theresa Kuhn, Janne Solgaard Jensen and Juliane Klein. (2011). The Europeanisation of Everyday Life: Cross-Border practices and Transantional Identitifcations Among the Eu and Third-Country Citizens. Foweler, J. B. a. N. (1997).Europeanization. Annual Review. Retrieved from http://www. jstor. org/stable/2952532 . Fukuyama, F. (2012). European Identities Retrieved from http://blogs. the-american-interest. com/fukuyama/2012/01/10/european-identities-part-i/ Margaret R, A. (2008). Perceptions of European Identity among EU Citizens: An Empirical Study. McCormick, J. (2010). Europeanism: Oxford University Press. Medrano, J. D. (2010). Unpackiing European Identity: CAIRN, INFO. Smith, A. D. (1993). National identity: Univeristy of Nevada Press. Versteegh, M. L. C. (2010). European Ci tizenship as a New Concept for Euroepan Identity.